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National Center for the Study and the Maintenance of the Forest Biodiversity of Peri

Logo del CNBF di Peri
 
  1. Laboratory for molecular biology
  2. Forest seeds analysis laboratory - ITML0600

The tree seed kiln of Peri

Lo stabilimento del CNBF di Peri

In the early seventies, the public administration responsible for the forests decided to build a new establishment for the production of forest seeds in the province of Verona, in the town of Peri. A public nursery, which had already been present in this area for decades, was then converted into the primary centre with the latest modern technology available. Over this first period it was used to regenerate mountain forests and the materials produced went to the nurseries of the alpine foresters.
The production of conifer seeds lost its importance and the attention was gradually redirected towards the need of tree replanting on the lowlands to the point that it became the most important activity. The Peri centre had to deal with new emergencies and had to, once more, adapt itself: it began to produce broad-leaved seeds for the plain territories, research new harvest areas and locate the species of the Po area thus no longer limiting its reseatch to alpine zones. The production line underwent some changes; it went from dealing with massive industrial quantities of few species to lesser quantities of many different species. The new productive reality enabled the selection of species present in nature in small quantities with a low seed yield.

In 2001, the centre was recognized as a National Centre for the study and conservation of the biodiversity of the forests (CNBF).
The study of the genetics of the forest species of the Po Plains was almost contemporarily adopted, thanks to the acquisition of a lab for molecular biology.

In 2005, it was accredited as a Forest Seed Analysis Laboratory by ISTA (International Seed Testing Association). The seed analysis certifications follow the ISTA (International Seed Test Association) guidelines.

The CNBF of Peri is a member of BIOFORV project research team promoting standard procedures related to the production of forest propagation (nursery). 

 
 
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Laboratory for the molecular biology

The areas of interest and the themes studied in this Centre concentrate on the understanding and on the evaluation of the genetic diversity of the forest species.
Most of the work and research is primarily focused on trees from the plains, more precisely, on the trees for which conservation is important since they are greatly tied to the preservation of the ecosystem.

With this objective in mind, it is important to emphasize the phylogeographical aspects of the species studied. In fact, the post-glacial reforestation (around 12,000 years ago) is the link between the genetic differences that are now observable within our forests. The reconstruction of the formation pattern of today's natural species allows us to locate possible areas containing relevant genetic peculiarity (e.g. refuge areas during the glacial era, areas with a consistent level of genetic variability, etc...). It is thus evident that the proper identification and conservation of these species represents a fundamental step for the study of intraspecific biodiversity

Some of our current projects are directed towards the understanding of the reproductive biology of the angiosperm and gymnosperm species. The objective of our researches is to understand the mechanisms and the processes that regulate the distribution of biodiversity within the natural specie formations. The outcome of these researches has enabled us to develop strategies regarding the collection of the propagation materials, taking also into account how the genetic diversity level is distributed throughout the forest, and, thus, enabling us to attain a more substantial evaluation of the total genetic diversity.
We also intend to clarify whether or not the obtainable data could be useful for the extrapolation of a seed harvest model that could then be adapted to forest formations of different structures or whether certain types of forest formations manifest such peculiarities that an ad hoc safeguarding plan could be implemented.

A third aspect to be considered when conducting research is the study and the diffusion of alien species, which are present in the natural environment. The objective of these researches is to monitor how an alien gene flow can influence the dynamism of the natural species of the forests. It will then be possible to obtain guidelines regarding the conservation strategies of specific natural environments previously submitted to a forest management plan that did not always exercise the necessary caution.

 
 
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Forest seeds analysis laboratory - ITML0600

Attivitą di selezione dei semi

The first analyses date back to 1977; the analyses were then based on humidity, purity, weight and germability of the forest species only, especially conifers (Abies, Pinus, Larix). Since 1994 the analyses started have been done using the technique of Tetrazolo Salts as the centre's main activity has now changed and, when under analysis, many species, like shrubs and trees, fell into dormancy. Besides, in 1994 the systematic data entry system was installed. Previous to this, all the necessary information needed for the proof reading of the analyzed species had to be recordet  in a notebook. At the time, there were no laws or decrees for the protocol of the analysis of treated seeds. Since November 2003, the quality system of the lab has undergone some structural changes. It is now required to write an instruction manual  the with procedures to follow and to keep a "workbook" to record what is being done. In 2005, the laboratory was accredited by the ISTA (International Seed Testing Association), an association that sets  the standardization criteria of the analytical methods to be followet within seed analysis laboratories. It is important to note that it was the only accredited lab of the forest department on a national level.

Moisture levels
The water levels contained within the seeds are determined through the use of a thermal scale; this parameter is important because it can determine the optimal moisture level needed to preserve the seeds after the mechanical selection has occurred.

Purity
The purity analysis is needed in order to determine the percentage of the composition in terms of the weight of a sample. In order to obtain this data, three main components have to be analyzed:
-Pure seed: the seed identified on the tag and accompanied by the sample.
-Other seeds: species of seeds that are different to the pure seed.
-Inert material: all that which isn't considered a seed (soil, wood, fruits...).

Weight of 1,000 seeds
The number of seeds in a certain weighed quantity are counted and then the weight of 1,000 seeds is  proportionally calculated. This qualitative parameter is important for the nurseries in order to enable  them to evaluate the number of seeds present in one kilo and, thus, determine how many little plants would grow from a batch.

Germinability
Germinability is the ability to determine to what extent a newborn germ will be able to develop into a normal plant under favorable conditions through the analysis of the aspects of its essential structures. The objective of the germability test is to determine the maximum germinative potential that can be obtained from a batch of seeds under standard conditions in terms of temperature, light and humidity on a substrate (paper, sand, terrain), and to compare the test results obtained from the different seed batches.

Tetrazolo salt test
In order to solve the dormancy problem, which requires  germability tests taking a very long time, even up to six months, the vitality test of a seed is applied through the Tetrazolo Salt technique. This test allows for a rapid evaluation of the vitality of a seed sample even when there is no direct correspondence between a live seed and a germinating one. The analysis is based on the results of the colour of the internal tissues of the seed when immersed in a salty colouring solution: the tissues of the vital seeds become red while those of the non-vital seeds do not change.

 
 
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Establishment production forest seeds
Via del Ponte, 256 37020 PERI (VR)
Tel: 045/6284071
Fax: 045/6284089
Email: utb.peri@corpoforestale.it

Contacts
Management
Office Manager: V.Q.A.F. Dr Franco Mason
Director - CNBF Manager V.Q.A.F. Dr Fabio Gorian

Forest seeds analysis laboratory - ITML0600
Laboratory Manager V.Q.A.F. Dr Fabio Gorian
Quality System Manager Sergio Pasquini

Genetic laboratory
Molecolar biologist Bruno Bertolasi
Molecolar biologist Luisa Zago

Seed sales office
Accounting Manager Isp. Capo Giuliano Da Zanche

Plant sales office
Assigned Lorenzo Gui
Assigned Simone Castelletti

Nursery
Manager Rev. Sergio Melchiori

Administration
Accounting manager Isp C. Giacomo Bolego